OR27 - Bacterial Chemotaxis and Biofilm Formation Genes in Primary and Secondary Endodontic Infections: A Whole-Metagenome Shotgun-Based Study

Abstract:
To identify genes encoding the bacterial chemotaxis and biofilm formation pathway in primary and secondary root canal infections using whole-genome shotgun sequencing, 37 samples from patients with primary and secondary root canal infections were evaluated using shotgun sequencing. The distribution was assessed of bacterial species and functional genes, including genes encoding for chemotaxis and biofilm pathway. Libraries were made using 1/4 Nextera XT reactions, and sequencing was done on the Novaseq with a target of 15-20M reads per sample. The Mann-Whitney U-test was used to compare differences in gene abundance between primary and secondary infections. The study identified 19 chemotaxis and 77 biofilm genes. Prevalent bacteria with chemotaxis genes were Fusobacterium nucleatum (62%), Pseudopropionibacterium propionicum, Selenomonas sputigena (10.8%), Desulfobulbus oralis (8.1%), and Campylobacter curvus. Top chemotaxis genes included K10540: methyl-galactoside transport system substrate-binding protein 70%, K10439 ribose transport system substrate-binding protein 56%, K03413: two-component system, chemotaxis family, response regulator CheY 51%, K02556: chemotaxis protein MotA 43%, K02557 chemotaxis protein MotB 37%. The prevalent bacteria encoding biofilm genes were Fusobacterium nucleatum, Desulfobulbus oralis, Campylobacter curvus (24.3%), Selenomonas sputigena (13%), and Eubacterium infirmum (43%). The top biofilm-related genes were K00688: starch phosphorylase (30/37) 81%, K00975: glucose-1-phosphate adenylyltransferase 81%, K03092: RNA polymerase sigma-54 factor 62%, K07173: S-ribosylhomocysteine lyase 64%, K00640: serine O-acetyltransferase 62%. Heatmaps illustrated the abundance of these genes in the samples. Bacteria carrying chemotaxis and biofilm pathway genes included Fusobacterium nucleatum, Pseudopropionibacterium propionicum, Selenomonas sputigena, Desulfobulbus oralis, and Campylobacter curvus. The study provides valuable insights into potential targets for future research and treatment in root canal infections.