PB-8 - Cell-based Regenerative Endodontics in Mature Teeth: Translation from Bench to Clinical Practice

Cell-free regenerative endodontic therapy (CF-RET) for mature teeth with apical periodontitis is a potential treatment alternative to traditional endodontic procedures based on clinical outcomes. However, there is increasing evidence that the tissues formed are “repaired” fibrous connective tissue including periodontal ligament, cementum, and bone-like tissues. On the other hand, the advantages of cell-based regenerative endodontic therapy (CB-RET) using exogenously processed mesenchymal stem cells are potentially to regenerate true pulp-dentin complex accompanied by reinnervation, revascularization, normal homeostatic and immunomodulatory functions. Application of CB-RET for mature teeth with apical periodontitis is a critical challenge to reestablish tooth vitality and function, leading to long-term endurance of the teeth. The aim of this presentation is to comprehensively review the advances in research of CB-RET in mature teeth with apical periodontitis in animal models and the translation to clinic. In this presentation, we will discuss two subjects: i) a clinically applicable protocol including an efficient irrigation protocol that disinfects completely and renders the microenvironment of the root canal space conducive to regeneration by stem cells, and ii) the predictability factors for a successful pulp regeneration.