(PO03) Natural Active Ingredients in Endodontic Reparative Materials: Synthesis, Comparative Evaluation, and Potential Applications

Abstract: Curcumin and chrysin, ancient therapeutic agents renowned for their antimicrobial, anti-inflammatory, and antioxidative properties, have garnered attention in the field of dentistry. Effective endodontic reparative materials must eradicate infections, curb acute inflammation, and induce mineralization to create an environment conducive to pulp tissue regeneration and healing. This study focused on synthesizing and comparing the antibacterial, anti-inflammatory, and alkaline phosphatase (ALP) activity of curcumin and chrysin-loaded endodontic reparative materials on human dental pulp stem cells (hDPSCs). Scaffolds, namely PCL (Poly-ɛ-caprolactone)-Gelatin-Chrysin (PCL-Gel-CH) and PCL-Gel-Curcumin (PCL-Gel-CUR), were meticulously synthesized and assessed. Their morphology was analyzed using scanning electron microscopy (SEM), while their antimicrobial properties were determined through the agar diffusion method. Both materials exhibited significant anti-inflammatory effects on inflamed hDPSCs, with curcumin-loaded scaffolds proving particularly effective against lipopolysaccharide (LPS)-induced inflammation. The ALP activity assay revealed substantial enzyme secretion in hDPSCs cultured on these materials, with curcumin-loaded scaffolds outperforming chrysin-loaded ones in enhancing ALP levels. In conclusion, curcumin and chrysin, when incorporated into the PCL-Gel scaffold, demonstrated noteworthy biological effects on hDPSCs. Their promising outcomes underscore their potential as natural and appealing ingredients for developing advanced endodontic reparative materials. This research paves the way for innovative approaches in endodontic therapy, potentially revolutionizing pulp tissue regeneration techniques.