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(PO110) Quantitative Analysis of Biofilm Removal Following Irrigation with EndoActivator and GentleWave Systems: Microbiological Study

Thursday, April 18, 2024
1:00 PM – 4:00 PM PT
Location: Level 1, Concourse Hall
Abstract: Enterococcus faecalis from the mesial canals and isthmuses of mandibular molars. Thirty extracted human lower molars were inoculated with E. faecalis OG1RF for 14 days, and then an initial bacterial sample was collected with paper points from mesio-buccal and mesio-lingual canals and isthmuses. The specimens were randomly divided into four groups (n=10 teeth; 20 canals each), according to irrigation systems: EndoActivator and GentleWave. The remaining ten teeth were divided between positive control, inoculated teeth without instrumentation or irrigation, and negative controls, teeth without inoculation. After instrumentation, the final sample was taken using paper points, and DNA was isolated. Primers specific for E. faecalis were used for qPCR. The bacterial reduction between pre- and post-instrumentation was calculated. One-way analysis of variance (ANOVA) with Tukey post hoc tests was used for statistical analysis with a significance of (p < .05). Both irrigation systems were able to reduce the load of E. faecalis from the prepared root canals. There was no significant difference between the effectiveness of the EndoActivator and the GentleWave in removing E. faecalis from the mesial canals and isthmuses of mandibular molars.