(PO117) The Efficacy of a New Modified EndoVac Irrigation Protocol

Abstract: The Sleiman’s Sequence is a new modified EndoVac irrigation protocol that uses the EndoVac tubing system with twice the quantity of solution. This study evaluated the efficacy of Sleiman’s Sequence in biofilm removal in single canaled teeth by comparing the amount of bacteria present before and after irrigation using the manufacturer’s recommended protocol. Forty extracted single canaled teeth were standardized to 20 mm in length, accessed, and instrumented to a 35/0.04 apical preparation size, using Vortex Blue NiTi rotary files. After sterilizing the teeth, they were infected with E. faecalis. After four weeks of incubation, a sample was collected prior to irrigation (S1). The teeth were then randomly divided into 4 groups with different irrigation protocols: (1) Conventional EndoVac (control); (2) Sleiman’s Sequence; (3) Passive Ultrasonic; and (4) Brasseler’s Total Vac. Irrigation protocols were performed with cycles of 0.5% NaOCl and 17% EDTA. An S2 sample was collected after each irrigation protocol. CFU counts from S1 and S2 were compared to evaluate the efficacy of each irrigation system. A one-way ANOVA test was used to analyze the data statistically. All groups showed a bacterial load reduction before and after their respective protocol. For Groups 1, 2, and 3, there were no significant differences in bacterial reduction (p > .05). Group 4 had significantly less bacterial reduction when compared to the other three groups (p < .05).Increasing the volume of solution used with the conventional EndoVac system did not result in increased efficacy of bacterial removal in this study.